biotechnological reduction of tobacco (nicotiana tabacum l.) toxicity
Authors
abstract
background: nicotiana tobacco contains large amounts of alkaloid nicotine. tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. utilizing the new methods of genetic engineering can modify nicotine levels in the plant. in this study, through transferring the blocking gene, the pathway of nicotine biosynthesis was blocked to produce transgenic tobacco with low levels of nicotine. methods: transgenic plants carrying t dna, and non-transgenic plants were grown on ms medium. then their leaves were dried and powdered. the plants were extracted with alkali solution. eventually, the nicotine content of the extract were analyzed using gc. results: the analysis of extracts showed a reduction in the nicotine content of the transgenic plant (contain t-dna) in comparison with non-transgenic plants. conclusion: tobacco with lower nicotine reduction can reduce the toxic effects of smoking on smokers and can facilitate withdrawal from it.
similar resources
Biotechnological Reduction of Tobacco (Nicotiana Tabacum L.) Toxicity
BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new ...
full textCoproporphyrinogenase in tobacco (Nicotiana tabacum L.).
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
full textMonograph of Tobacco (nicotiana Tabacum)
The use of tobacco dates back to the ancient civilizations of the Americas, where it played a central role in religious occasions. The peoples smoked tobacco in cigars and pipes and chewed it with lime, for its pleasurable euphoriant effects. In the 16 th century, Europeans spread the use of tobacco in North America, while the Spanish bring it into Europe. In the 1559, Jean Nicot, the French am...
full textHigh-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
full textGenetic Study of Agronomic Traits in Tobacco (Nicotiana tabacum L.) Using Hayman’s Graphical Analysis of Diallel
For the purpose of graphical analysis and estimation of genetic parameters related to yield and its components, six tobacco genotypes was crossed in half -diallel mating design. In this study, the F2 progenies and their parents were evaluated in a randomized complete block design with three replications. The results of analysis of variance indicated a significant difference between genotypes fo...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of toxicologyجلد ۶، شماره ۱۸، صفحات ۶۹۹-۷۰۳
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023